Dye Alternatives

Waterborne, Inc. offers an extensive line of fluorescent antibody kits, with a variety of fluorochromes, for use in the detection of cysts, oocysts, and spores of Giardia spp., Cryptosporidium spp., and microsporidian species (Encephalitozoon intestinalis, E. cuniculi, E. hellem, and Enterocytozoon bienusi) in water, food, and various environmental samples, and for use in veterinary clinical diagnosis.

Detection kits can be used in the following manner:

  • immunofluorescence microscopy
  • flow cytometry
  • fluorescence-activated cell sorting
  • laser scanning-based systems
  • immunomagnetic separation (IMS methods).

 
Detection kits for water and food testing are available for the simultaneous detection of Giardia and Cryptosporidium, containing combined antibodies against each parasite or as individual antibody kits for the detection of single parasite stages, genera, or species.

A variety of fluorochromes are available in the detection kits as seen in the chart below.*

Fluorochrome Fluorochrome Code Fluorescence Maximum l (nm)
Excitation Emission
Aminomethylcoumarin AMCA Blue 352 448
AlexaFluor350 AF350 Blue 346 442
Fluorescein (FL-5-EX) FL Green 492 516
Alexa Fluor 488 AF488 Green 495 519
Tetramethylrhodamine TAMRA Red-Orange 546 580
Cy3 Cy3 Red-Orange 550 570
Alexa Fluor 555 AF555 Red-Orange 555 565
R-phycoerythrin RPE Red 480/546/565 578
Texas Red TR Red 583 603
Cy5 Cy5 Deep Red* 640 670
Alexa Fluor 647 AF647 Deep Red* 650 668

*Not visible with use of filter set.  For use with flow cytometry only.

**If you do not see a specific fluorochrome listed, please let us know.