A100FLACK. Aqua-Glo™ G/C Direct Comprehensive Kit with AccuSpike™-IR

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A100FLACK. Aqua-Glo™ G/C Direct Comprehensive Kit with AccuSpike™-IR

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The Aqua-Glo kit is designed to detect the cyst and oocyst stages of Giardia lamblia and Cryptosporidium parvum in particulates isolated from water and other environmental samples utilizing the principle of direct immunofluorescence. 

The antibody reagent consists of a mixture of fluorescein-labeled mouse monoclonal antibodies made to cyst and oocyst outer wall antigenic sites (epitopes) of Giardia lamblia and Cryptosporidium parvum. This reagent is genus-specific and will bind only to the cysts and oocysts of these two parasites if they are present.  The reagent shows varying degrees of cross-reactivity with cysts and oocysts of other species of Giardia and Cryptosporidium.  The cysts and/or oocysts will appear bright apple green when viewed under a fluorescence microscope using the appropriate filters for fluorescein. This antibody cross-reacts with some species of algae.

AccuSpike-IR vials (12) are designed for percent recovery determination with matrix (environmental) and reagent water samples.  Each 2.0 mL polypropylene vial contains a count, by flow-cytometry cell sorting, of 100 each of G. lamblia cysts and C. parvum oocysts.  The organisms are suspended in 0.75 mL de-ionized water with 0.01% Tween 20, and have been inactivated by gamma irradiation. The internal structures of the cysts and oocysts are intact and easily identified, and the cells are fully reactive with antibody-coated immunomagnetic particles and fluorescent antibodies.  Although they are non-infectious, as proven by mouse inoculation and in vitro monolayer inoculation experiments, proper laboratory procedures and personal protective equipment are recommended as per US EPA Methods 1622, 1623, and 1623.1.

Product Instructions

SDS AccuSpike-IR

SDS A100FLR

Certificate of Analysis AccuSpike-IR

Certificate of Analysis A100

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Kit Includes:

  • A100FLR-1X: 1 dropper vial containing 3.5 mL working dilution (1x) reagent
  • PC101: 1 glass vial containing 1 mL positive control
  • WB101: 1 screw cap bottle containing 50 mL 1x SureRinse Wash Buffer
  • C101: 1 dropper vial containing 3.5 mL BlockOut counterstain
  • D101: 1 microtube containing 0.4 mL DAPI, 5000X in methanol
  • M101: 1 dropper vial containing 3.5 mL No-Fade Mounting Medium
  • S100-2: 1 box of two-well SuperStick Slides, 40/box
  • PACIR12: AccuSpike-IR, G/C Quality Control Standard, 12 vials