Dye Alternatives
Waterborne™, Inc. offers an extensive line of fluorescent antibody kits, with a variety of fluorochromes, for use in the detection of cysts, oocysts, and spores of Giardia spp., Cryptosporidium spp., and microsporidian species (Encephalitozoon intestinalis, E. cuniculi, E. hellem, and Enterocytozoon bienusi) in water, food, and various environmental samples, and for use in veterinary clinical diagnosis.
Detection kits can be used in the following manner:
immunofluorescence microscopy
flow cytometry
fluorescence-activated cell sorting
laser scanning-based systems
immunomagnetic separation (IMS methods).
Detection kits for water and food testing are available for the simultaneous detection of Giardia and Cryptosporidium, containing combined antibodies against each parasite or as individual antibody kits for the detection of single parasite stages, genera, or species.
A variety of fluorochromes are available in the detection kits as seen in the chart below.*
Fluorochrome | Fluorochrome Code | Fluorescence | Maximum l (nm) | |
---|---|---|---|---|
Excitation | Emission | |||
AlexaFluor350 | AF350 | Blue | 346 | 442 |
Fluorescein (FL-5-EX) | FL | Green | 492 | 516 |
Alexa Fluor 488 | AF488 | Green | 495 | 519 |
Cy3 | Cy3 | Red-Orange | 550 | 570 |
Alexa Fluor 555 | AF555 | Red-Orange | 555 | 565 |
Texas Red | TR | Red | 583 | 603 |
Cy5 | Cy5 | Deep Red* | 640 | 670 |
Alexa Fluor 647 | AF647 | Deep Red* | 650 | 668 |
*Not visible with use of filter set. For use with flow cytometry only.
**If you do not see a specific fluorochrome listed, please let us know.