The Crypt-a-Glo™ kit is designed to detect the oocyst stage of Cryptosporidium parvum in particulates isolated from water and other environmental samples utilizing the principle of direct immunofluorescence.

The antibody reagent consists of a fluorescein-labeled mouse monoclonal antibody made to oocyst outer wall antigenic sites (epitopes) of Cryptosporidium parvum. This reagent is genus-specific and will bind only to the oocysts if they are present. The reagent shows varying degrees of cross-reactivity with oocysts of other species of Cryptosporidium. The oocysts will appear bright apple green when viewed under a fluorescence microscope using the appropriate filters for fluorescein. This antibody cross-reacts with some species of algae.

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