A400FLR-20X Crypt-a-Glo™ Reagent Only Kit

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A400FLR-20X Crypt-a-Glo™ Reagent Only Kit

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The Crypt-a-Glo™ reagent is designed to detect the oocyst stage of Cryptosporidium parvum utilizing the principle of direct immunofluorescence. It is not meant for detection of Cryptosporidium life cycle stages other than the oocyst. 

The antibody reagent included consists of a fluorescein-labeled mouse monoclonal antibody made to an oocyst wall antigenic site (epitope) of Cryptosporidium parvum.  The reagent shows varying degrees of cross-reactivity with oocysts of other species of Cryptosporidium.  The oocysts will appear bright apple-green when viewed under a fluorescence microscope using the appropriate filters for fluorescein.  The reagent is a 20x concentrated solution and provides enough reagent for over 400 tests, using one drop per test (approximately 45 uL per drop.)  The antibody reagent contains 0.04% w/v sodium azide as preservative and 1% bovine serum albumen as antibody stabilizer.

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Kit Includes

  • A400FLR-20X: 1 glass vial containing 1.0 mL 20x concentrated reagent.
  • This solution must be diluted to 1x before use.
  • B100-20: 1 screw cap bottle containing 20 mL Dilution/Blocking (DB) Buffer
  • PC101: 1 glass vial containing 1 mL positive control
  • M101: 1 dropper vial containing 3.5 mL No-Fade Mounting Medium