The Sporo-Glo™ kit utilizes the principle of direct immunofluorescence.
The reagent consists of a fluorescein-labeled rat anti-Cryptosporidium parvum sporozoite polyclonal antibody. The reagent will bind to sporozoites, merozoites, and all other intracellular reproductive stages; these will appear bright apple-green when viewed under a fluorescence microscope using the appropriate filters for fluorescein. Reagent included is a working dilution (1x) of fluorescein (FL)-labeled polyclonal rat IgG antibody made against sporozoites of the Iowa bovine isolate of Cryptosporidium parvum. The antibody reagent contains 0.04% w/v sodium azide as preservative and 1% bovine serum albumen as antibody stabilizer. This reagent reacts more strongly with the intracellular dividing stages than it does with sporozoites. There is some degree of cross-reactivity of this reagent with oocyst walls.
- A600FLR-1X: 1 dropper vial containing 3.5 mL working dilution (1x) reagent