The Sporo-Glo™ kit utilizes the principle of direct immunofluorescence. The reagent consists of a fluorescein-labeled rat anti-Cryptosporidium parvum sporozoite polyclonal antibody. The reagent will bind to sporozoites, merozoites, and all other intracellular reproductive stages; the specimens will appear bright apple-green when viewed under a fluorescence microscope using the appropriate filters for fluorescein.
The reagent included is a concentrated solution (20x) of fluorescein (FL)-labeled polyclonal rat IgG antibody made against sporozoites of the Iowa bovine isolate of Cryptosporidium parvum. The antibody reagent contains 0.04% w/v sodium azide as preservative and 1% bovine serum albumen as antibody stabilizer. This reagent reacts more strongly with the intracellular dividing stages than it does with sporozoites. There is some degree of cross-reactivity of this reagent with oocyst walls.
- A600FLR-20X: 1 glass vial containing 1.0 mL 20x concentrated reagent solution
- This solution must be diluted to 1x before use
- B100-20: 1 screw cap bottle containing 20 mL Dilution/Blocking (DB) Buffer
- M101: 1 dropper bottle vial containing 3.5 mL No-Fade™ Mounting Medium