A600FLR-20X Sporo-Glo™

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A600FLR-20X Sporo-Glo™

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The Sporo-Glo kit utilizes the principle of direct immunofluorescence.  The reagent consists of a fluorescein-labeled rat anti-Cryptosporidium parvum sporozoite polyclonal antibody.  The reagent will bind to sporozoites, merozoites, and all other intracellular reproductive stages; the specimens will appear bright apple-green when viewed under a fluorescence microscope using the appropriate filters for fluorescein. 

The reagent included is a concentrated solution (20x) of fluorescein (FL)-labeled polyclonal rat IgG antibody made against sporozoites of the Iowa bovine isolate of Cryptosporidium parvum.  The antibody reagent contains 0.04% w/v sodium azide as preservative and 1% bovine serum albumen as antibody stabilizer.  This reagent reacts more strongly with the intracellular dividing stages than it does with sporozoites.  There is some degree of cross-reactivity of this reagent with oocyst walls. 

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Kit Includes:    

  • A600FLR-20X: 1 glass vial containing 1.0 mL 20x concentrated reagent solution
  • This solution must be diluted to 1x before use
  • B100-20: 1 screw cap bottle containing 20 mL Dilution/Blocking (DB) Buffer
  • M101: 1 dropper bottle vial containing 3.5 mL No-Fade Mounting Medium